Virus Influenza beku-garing/Virus Influenza B Asam Nukléat
Ngaran produk
HWTS-RT193-Virus Influenza beku-kering/Virus Influenza B Kit Detéksi Asam Nukléat (Fluoresensi PCR)
Épidemiologi
Nurutkeun bédana antigen antara gén NP jeung M, virus influenza bisa dibagi jadi opat jenis: virus influenza A (IFV A), virus influenza B (IFV B), virus influenza C (IFV C) jeung virus influenza D (IFV D). Pikeun virus influenza A, virus ieu ngagaduhan seueur host sareng serotipe kompleks, sareng tiasa nyebarkeun ka host ngalangkungan rékombinasi genetik sareng mutasi adaptif. Manusa kakurangan kekebalan anu tahan ka virus influenza A, ku kituna jalma-jalma tina sagala umur umumna rentan. Virus influenza A nyaéta patogén paling utama anu nyababkeun pandém influenza. Pikeun virus influenza B, biasana nyebar di daérah leutik sareng ayeuna teu aya subtipe. Inféksi manusa utamana disababkeun ku virus influenza turunan B/Yamagata atawa B/Victoria. Diantara kasus influenza anu dikonfirmasi bulanan di 15 nagara di daérah Asia-Pasifik, tingkat diagnosis virus influenza B antara 0 dugi ka 92%. Beda sareng virus influenza A, kelompok jalma anu tangtu, sapertos murangkalih sareng manula, rentan ka virus influenza B, anu tiasa nyababkeun komplikasi, bahkan langkung beban ka masarakat tibatan virus influenza A.
Parameter Téknis
| Panyimpenan | 2-28℃ |
| Kahirupan rak | 12 bulan |
| Jenis spésimén | Ngusap tikoro |
| Ct | IFV A,IFVB Ct≤35 |
| CV | <5,0% |
| LoD | 200 Salinan/mL |
| Spésifikasi | Cross-réaktivitas: Teu aya cross-réaktivitas antara kit sareng Bocavirus, Rhinovirus, Cytomegalovirus, Respiratory syncytial virus, Parainfluenza virus, Epstein-Barr virus, herpes simplex virus, varicella-zoster virus, Mumps virus, Enterovirus, Measles virus, human metapneumoviruses, coronavirus, Adenovirus, MVP-CoV Rotavirus, Norovirus, Chlamydia pneumoniae, Mycoplasma pneumoniae, Streptococcus pneumoniae, Klebsiella pneumoniae, Streptococcus pyogenes, Legionella, Pneumocystis jirovecii, Haemophilus influenzae, Bordetella pertussis, Staphylococcus aureus, Mycobacterium tuberkulosis, Mycobacterium tuberkulosis, Candida Candida, Neurobakteria. glabrata, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrhalis, Lactobacillus, Corynebacterium jeung DNA génomik manusa. Uji gangguan: Mucin (60mg/mL), getih manusa (50%), Phenylephrine (2 mg/mL), Oxymetazoline (2mg/mL), Natrium klorida (20mg/mL) kalawan 5% pengawet, Beclomethasone (20mg/mL), Dexamethasone (20mg/mL), Trinisoliglone (2mg/mL), Budesonide (1mg/mL), Mometasone (2mg/mL), Fluticasone (2mg/mL), Histamine hydrochloride (5 mg/mL), Benzocaine (10%), Menthol (10%), Zanamivir (20mg/mL), Peramivir (1mg/mL), Mupiromcin (20mg/mL), Mupiromcin/L. Oseltamivir (60ng/mL), Ribavirin (10mg/L) dipilih pikeun uji interferensi, sarta hasil némbongkeun yén zat interfering dina konsentrasi luhur teu boga réaksi interferensi kana hasil tés kit. |
| Instrumén lumaku | Lumaku pikeun réagen tés Tipe I: Applied Biosystems 7500 Real-Time PCR Systems, SLAN-96P Real-Time PCR Systems (Hongshi Médis Téhnologi Co., Ltd.). Lumaku pikeun réagen tés Tipe II: EudemonTM AIO800 (HWTS-EQ007) ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Aliran Gawé
PCR konvensional
Makro & Micro-Test Umum DNA / RNA Kit (HWTS-3019) (anu tiasa dianggo sareng Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, (HWTS-3006B)) ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Disarankeun pikeun ékstraksi sampel saluyu sareng substitusi Kit FU.
AIO800 sadaya-dina-hiji mesin
