Salapan Jenis Virus Pernafasan
Ngaran produk
HWTS-RT185A-Salapan Jenis Virus Pernafasan Kit Deteksi Asam Nukléat (Fluoresensi PCR)
Épidemiologi
Inféksi saluran pernapasan mangrupikeun jinis panyawat manusa anu paling umum, anu tiasa lumangsung dina sagala jenis kelamin, umur sareng daérah, sareng mangrupikeun salah sahiji panyabab utama morbiditas sareng maot di dunya.[1]. Patogen pernapasan anu umum sacara klinis kalebet virus influenza A (IFV A), virus influenza B (IFV B), coronavirus novel (SARS-CoV-2), virus respiratory syncytial, adenovirus, metapneumovirus manusa, rhinovirus, virus parainfluenza (I/II/III) sareng mycoplasma pneumoniae, jsb.[2,3]. Gejala klinis sareng tanda anu disababkeun ku inféksi saluran pernapasan relatif sami, tapi inféksi anu disababkeun ku patogén anu béda-béda gaduh metode perawatan anu béda, épék kuratif sareng kursus panyakit.[4,5]. Ayeuna, metodeu utama deteksi laboratorium patogén pernapasan kalebet: isolasi virus, deteksi antigen sareng deteksi asam nukléat. Kit ieu ngadeteksi sareng ngidentipikasi asam nukléat virus khusus dina individu anu ngagaduhan tanda sareng gejala inféksi pernapasan, sareng hasil klinis sareng laboratorium sanés pikeun ngabantosan diagnosis inféksi virus pernapasan.
Saluran
| FAM | asam nukléat MP |
| ROX | Kontrol internal |
Parameter Téknis
| Panyimpenan | 2-8 ℃ |
| Kahirupan rak | 12 bulan |
| Jenis spésimén | swab oropharyngeal; swab nasopharyngeal |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5,0% |
| LoD | 200 Salinan/mL |
| Spésifikasi | Réaktivitas silang: Teu aya réaktivitas silang antara kit sareng virus Boca, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptoellacoccus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, Klebsigene Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Corynebaclicus DNA. Uji interferensi: Pilih mucin (60mg/mL), getih manusa (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), natrium klorida kalawan pengawet 5% (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), dexamethasone (20mg/mL). (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), mentol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mucinpirocin (20mg/L), mucinpirocin (20mg/L) oseltamivir (60ng/mL), ribavirin (10mg/L), Hasilna nunjukkeun yén zat anu ngaganggu dina konsentrasi di luhur teu aya réaksi anu ngaganggu kana deteksi kit. |
| Instrumén lumaku | Lumaku pikeun réagen deteksi tipe I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time Sistim PCR (Hongshi Médis Téhnologi Co., Ltd.), LineGene 9600 Ditambah Real-Time Sistim deteksi PCR (FQD-96A, Hangzhou téhnologi Bioer), MA-6000 Real-Time kuantitatif Thermal Cycler (Suzhou Molarray RealTime CFXR, PCR, System) System. Opus 96 Real-Time Sistim PCR. Lumaku pikeun tipe II réagen deteksi: Eudemon TM AIO800 (HWTS-EQ007) ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
| Panyimpenan | 2-8 ℃ |
| Kahirupan rak | 12 bulan |
| Jenis spésimén | swab oropharyngeal; swab nasopharyngeal |
| Ct | COVID-9, IFV A, IFVB, RSV, Adv, hMPV, Rhv, PIV, MP Ct≤35 |
| CV | <5,0% |
| LoD | 200 Salinan/mL |
| Spésifikasi | Réaktivitas silang: Teu aya réaktivitas silang antara kit sareng virus Boca, Cytomegalovirus, Epstein-Barr virus, Herpes simplex virus, varicella zoster Virus, Mumps virus, Enterovirus, measles virus, human coronavirus, SARS Coronavirus, MERS Coronavirus, Rotavirus, Norovirus, Chlamydia pneumoniae, Streptoellacoccus pneumoniae, Streptococcus pneumoniae, Streptococcus pneumoniae, Klebsigene Pneumospora, Haemophilus influenzae, Bacillus pertussis, Staphylococcus aureus, Mycobacterium tuberculosis, gonococcus, Candida albicans, Candida glabra, Aspergillus fumigatus, Cryptococcus neoformans, Streptococcus salivarius, Moraxella catarrh, Corynebaclicus DNA. Uji interferensi: Pilih mucin (60mg/mL), getih manusa (50%), benephrin (2mg/mL), hydroxymethazoline (2mg/mL) 2mg/mL), natrium klorida kalawan pengawet 5% (20mg/mL), beclomethasone (20mg/mL), dexamethasone (20mg/mL), dexamethasone (20mg/mL). (2mg/mL), budesonide (1mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), benzocaine (10%), mentol (10%), zanamivir (20mg/mL), peramivir (1mg/mL), mucinpirocin (20mg/L), mucinpirocin (20mg/L) oseltamivir (60ng/mL), ribavirin (10mg/L), Hasilna nunjukkeun yén zat anu ngaganggu dina konsentrasi di luhur teu aya réaksi anu ngaganggu kana deteksi kit. |
| Instrumén lumaku | Lumaku pikeun réagen deteksi tipe I: Applied Biosystems 7500 Real-Time PCR Systems, QuantStudio®5 Real-Time PCR Systems, SLAN-96P Real-Time Sistim PCR (Hongshi Médis Téhnologi Co., Ltd.), LineGene 9600 Ditambah Real-Time Sistim deteksi PCR (FQD-96A, Hangzhou téhnologi Bioer), MA-6000 Real-Time kuantitatif Thermal Cycler (Suzhou Molarray RealTime CFXR, PCR, System) System. Opus 96 Real-Time Sistim PCR. Lumaku pikeun tipe II réagen deteksi: Eudemon TM AIO800 (HWTS-EQ007) ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. |
Aliran Gawé
Makro & Micro-Test Viral DNA/RNA Kit (HWTS-3017) (anu tiasa dianggo sareng Macro & Micro-Test Automatic Nucleic Acid Extractor (HWTS-3006C, HWTS-3006B)), sareng Macro & Micro-Test Viral DNA/RNA Kit (HWTS-3017-8)TM AIO800 (HWTS-EQ007)) ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd.
Volume sampel anu sasari nyaéta 200μL sareng volume élusi anu disarankeun nyaéta 150μL.
