4 Jenis Virus Pernafasan
Ngaran produk
HWTS-RT099- 4 Jenis Virus Pernafasan Kit Deteksi Asam Nukléat (Fluorescence PCR)-内参是NED-ABI 7500 Real-Time PCR System/ ABI 7500 gancang Real-Time PCR Systems / QuantStudio®5 Sistem PCR Real-Time
HWTS-RT158-4 Jenis Virus Pernafasan Kit Detéksi Asam Nukléat (Fluoresensi PCR) -内参Quasar 705
Épidemiologi
Kasakit Virus Corona 2019, disebut "COVID-19", nujul kana pneumonia disababkeun ku2019-nCoVinféksi.2019-nCoVmangrupa coronavirus milik genus β. COVID-19 mangrupikeun panyakit inféksi saluran pernapasan akut, sareng populasi umumna rentan. Ayeuna, sumber inféksi utamina pasien anu katépa ku2019-nCoV, sareng jalma anu katépaan asimtomatik ogé tiasa janten sumber inféksi. Dumasar kana panalungtikan epidemiologis ayeuna, jaman inkubasi nyaéta 1-14 dinten, kalolobaanana 3-7 dinten. Demam, batuk garing sareng kacapean mangrupikeun manifestasi utama. Sababaraha pasien ngagaduhan gejalas sapertoskamacetan nasal, irung runny, nyeri tikoro, myalgia sarta diare, jsb.
Saluran
| FAM | 2019-nCoV |
| VIC(HEX) | RSV |
| CY5 | IFV A |
| ROX | IFV B |
| NED | Kontrol internal |
Parameter Téknis
| Panyimpenan | -18 ℃ |
| Kahirupan rak | 9 bulan |
| Jenis spésimén | Oropharyngeal swab |
| Ct | ≤38 |
| LoD | 2019-nCoV: 300 Salinan/mLVirus influenza A/Virus Influenza B/Virus syncytial engapan: 500 Salinan/mL |
| Spésifikasi | a) Hasil cross-réaktivitas nunjukkeun yén teu aya réaksi silang antara kit sareng coronavirus manusa SARSr-CoV, MERSr-CoV, HCoV-OC43, HCoV-229E, HCoV-HKU1, HCoV-NL63, parainfluenza virus tipe 1, 2, 3, rhinovirus A, B, C, Aemochlamydia virus enterovirus, humanydia enterovirus B, C, D, virus pulmonal manusa, virus epstein-barr, virus cacar, virus cytomegalo manusa, rotavirus, norovirus, virus parotitis, virus varicella-zoster, legionella, bordetella pertussis, haemophilus influenzae, staphylococcus aureus, streptococcus pneumoniae, streptococcus, mycococcus pneumoniae, streptococcus, mycococcus pneumoniae. haseup aspergillus, candida albicans, candida glabrata, pneumocystis jiroveci jeung cryptococcus bayi jeung asam nukléat génomik manusa. b) Kamampuhan anti interferensi: pilih mucin (60mg/mL), 10% (v/v) getih jeung phenylephrine (2mg/mL), oxymetazoline (2mg/mL), natrium klorida (kaasup pengawet) (20 mg/mL), beclomethasone (20mg/mL), deksametason (20mg/mL), deksametason (20mg/mL) triamcinolone acetonide (2mg/mL), budesonide (2mg/mL), mometasone (2mg/mL), fluticasone (2mg/mL), histamine hydrochloride (5mg/mL), alpha interferon (800IU/mL), zanamivir (20mg/mL), ribavirin (10mg/mL), ribavirin (10mg/mL) (1mg/mL), lopinavir (500mg/mL), ritonavir (60mg/mL), mupirocin (20mg/mL), azitromisin (1mg/mL), ceftriaxone (40μg/mL), meropenem (200mg/mL), levofloxacin (10μg/mL) jeung for test. Hasilna nunjukkeun yén zat anu ngaganggu konsentrasi anu disebatkeun di luhur henteu gaduh réaksi gangguan kana hasil tés patogén. |
| Instrumén lumaku | Applied Biosystems 7500 Real-Time PCR SystemApplied Biosystems 7500 gancang Real-Time PCR Systems QuantStudio®5 Systems PCR Real-Time |
Aliran Gawé
Pilihan 1.
Makro & Micro-Test Viral DNA / RNA Kit (HWTS-3004-32, HWTS-3004-48, HWTS-3004-96) jeung Macro & Micro-Test Automatic Nukléat Asam Extractor (HWTS-3006) dihasilkeun ku Jiangsu Macro & Micro-Test Med-Tech 0μlu sampel volume jeung ékstrak The Med-Tech0 μL. volume nyaéta 80μL.
Pilihan 2.
QIAamp Viral RNA Mini Kit (52904) dihasilkeun ku QIAGEN atanapi Nukléat Asam ékstraksi atanapi purifikasi Kit (YDP315-R) dihasilkeun Tiangen Biotéh (Beijing) Co., Ltd. Volume sampel sasari téh 140μL, sarta volume elusi dianjurkeun nyaéta 60μL.
