14 Jenis Patogen Pernapasan Gabungan
Ngaran produk
HWTS-RT159B 14 Jenis Patogen Pernapasan Kit Deteksi Asam Nukléat Gabungan (PCR Fluoresensi)
Epidemiologi
Infeksi saluran pernapasan mangrupikeun panyakit anu paling umum dina manusa, anu tiasa kajantenan dina sagala jinis kelamin, umur sareng daérah. Éta mangrupikeun salah sahiji panyabab utama morbiditas sareng mortalitas dina populasi di sakumna dunya.[1]Patogén pernapasan umum kalebet coronavirus anyar, virus Influenza A, virus Influenza B, virus Respiratory syncytial, Adenovirus, human metapneumovirus, rhinovirus, virus Parainfluenza tipe I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, sareng Streptococcus pneumoniae, jsb.[2,3].
Saluran
| Posisi Sumur | Ngaran Larutan Réaksi | Patogén anu kedah dideteksi |
| 1 | Campuran Master A | SARS-CoV-2, IFV A, IFV B |
| 2 | Campuran Master B | Adv, hMPV, MP, Cpn |
| 3 | Campuran Master C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Campuran D | CoV, EV, SP, kontrol internal |
| 5 | Campuran Master A | SARS-CoV-2, IFV A, IFV B |
| 6 | Campuran Master B | Adv, hMPV, MP, Cpn |
| 7 | Campuran Master C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Campuran D | CoV, EV, SP, kontrol internal |
Parameter Téknis
| Panyimpenan | ≤-18℃ |
| Umur simpan | 9 bulan |
| Jenis Spésimen | Swab orofaring, swab nasofaring |
| Ct | ≤38 |
| CV | <5.0% |
| LoD | 200 Salinan/mL |
| Spésifikasi | Hasil uji cross-reactivity nunjukkeun yén teu aya cross reaction antara kit ieu sareng Cytomegalovirus, Herpes simplex virus type 1, Varicella-zoster virus, Epstein-Barr virus, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophila, Moraxella catarrhalis, attenuated strains of Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia cepacia, Corynebacterium striatum, Nocardia, Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii sareng asam nukléat génomik manusa. |
| Instrumen anu Lumaku | Sistem PCR Real-Time SLAN-96P (Hongshi Medical Technology Co., Ltd.)Sistem PCR Real-Time Biosystems Terapan 7500 Sistem PCR Real-Time Gancang Biosystems Terapan 7500 QuantStudio®5 Sistem PCR Real-Time Sistem Deteksi PCR Real-Time LineGene 9600 Plus (FQD-96A, téknologi Hangzhou Bioer) MA-6000 Siklus Termal Kuantitatif Waktos Nyata (Suzhou Molarray Co., Ltd.) Sistem PCR Waktos Nyata BioRad CFX96, Sistem PCR Waktos Nyata BioRad CFX Opus 96 |
Aliran Padamelan
Réagen ékstraksi anu disarankeun: Kit DNA/RNA Viral Macro & Micro-Test (HWTS-3017) (anu tiasa dianggo sareng Ékstraktor Asam Nukléat Otomatis Macro & Micro-Test (HWTS-EQ010)) ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Volume sampel anu diekstrak nyaéta 200µL. Léngkah-léngkah salajengna kedah dilakukeun numutkeun pitunjuk pikeun panggunaan réagen ékstraksi ieu. Volume élusi anu disarankeun nyaéta80µL.
