14 Jenis Patogén Pernafasan Gabungan
Ngaran produk
HWTS-RT159B 14 Jenis Patogén Pernapasan Gabungan Kit Detéksi Asam Nukléat (Fluoresensi PCR)
Épidemiologi
Inféksi saluran pernapasan mangrupikeun panyakit anu paling umum dina manusa, anu tiasa lumangsung dina sagala jenis kelamin, umur sareng daérah. Éta mangrupikeun salah sahiji panyabab utama morbiditas sareng mortalitas dina populasi sadunya[1]. Patogen pernapasan umum kalebet coronavirus novel, virus Influenza A, virus Influenza B, Virus syncytial Respiratory, Adenovirus, metapneumovirus manusa, rhinovirus, virus Parainfluenza tipe I/II/III/IV, Bocavirus, Enterovirus, Coronavirus, Mycoplasma pneumoniae, Chlamydia pneumoniae, sareng Streptococcus pneumoniae, jsb[2,3].
Saluran
| Posisi sumur | Ngaran Solusi Réaksi | Patogén pikeun dideteksi |
| 1 | Master Campur A | SARS-CoV-2, IFV A, IFV B |
| 2 | Master Campur B | Adv, hMPV, MP, Cpn |
| 3 | Master Campuran C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 4 | Master Campur D | CoV, EV, SP, kontrol internal |
| 5 | Master Campur A | SARS-CoV-2, IFV A, IFV B |
| 6 | Master Campur B | Adv, hMPV, MP, Cpn |
| 7 | Master Campuran C | PIVI/II/III/IV, Rhv, RSV, HBoV |
| 8 | Master Campur D | CoV, EV, SP, kontrol internal |
Parameter Téknis
| Panyimpenan | ≤-18 ℃ |
| Kahirupan rak | 9 bulan |
| Jenis spésimén | swab oropharyngeal, swab nasofaring |
| Ct | ≤38 |
| CV | <5,0% |
| LoD | 200 Salinan/mL |
| Spésifikasi | Hasil uji réaktivitas silang nunjukkeun yén teu aya réaksi silang antara kit ieu sareng Cytomegalovirus, virus Herpes simpléks tipe 1, virus Varicella-zoster, virus Epstein-Barr, Bordetella pertussis, Corynebacterium, Escherichia coli, Haemophilus influenzae, Lactobacillus, Legionella pneumophilalis, galur Legionella. Mycobacterium tuberculosis, Neisseria meningitidis, Neisseria, Pseudomonas aeruginosa, Staphylococcus aureus, Staphylococcus epidermidis, Streptococcus pyogenes, Streptococcus salivarius, Acinetobacter baumannii, Stenotrophomonas maltophilia, Burkholderia, Corstriactum, Corstriatum, No. Serratia marcescens, Citrobacter, Cryptococcus, Aspergillus fumigatus, Aspergillus flavus, Pneumocystis jiroveci, Candida albicans, Rothia mucilaginosus, Streptococcus oralis, Klebsiella pneumoniae, Chlamydia psittaci, Coxiella burnetii jeung inti génomik asam manusa. |
| Instrumén lumaku | SLAN-96P Real-Time PCR Systems (Hongshi Médis Téhnologi Co., Ltd.)Applied Biosystems 7500 Real-Time PCR Systems Applied Biosystems 7500 gancang Real-Time PCR Systems QuantStudio®5 Sistem PCR Real-Time LineGene 9600 Plus Real-Time PCR Deteksi Systems (FQD-96A, téhnologi Hangzhou Bioer) MA-6000 Real-Time Quantitative Thermal Cycler (Suzhou Molarray Co., Ltd.) BioRad CFX96 Real-Time PCR System, BioRad CFX Opus 96 Real-Time PCR System |
Aliran Gawé
Reagen ékstraksi dianjurkeun: Makro & Micro-Test Viral DNA / RNA Kit (HWTS-3017) (anu bisa dipaké kalawan Macro & Micro-Test Otomatis Nukléat Asam Extractor (HWTS-EQ010)) ku Jiangsu Macro & Micro-Test Med-Tech Co., Ltd. Volume sampel sasari nyaéta 200µL. Léngkah-léngkah saterasna kedah dilakukeun dumasar kana petunjuk pikeun ngagunakeun réagen ékstraksi ieu. Volume élusi anu disarankeun nyaéta80µL.
